Molecular Characterization of Trichoderma harzianum and Trichoderma viride Isolated from Rhizosphere Soils by RAPD Markers

Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles

Nineteen isolates of Trichoderma viride and Trichoderma harzianum obtained from rhizosphere soil of plantation crops, forest soil and agricultural fields of North Bengal region were studied using RAPD and ITS-PCR. The genetic relatedness among eleven isolates of T. viride and eight isolates of T. harzianum were analyzed with six random primers. RAPD profiles showed genetic diversity among the i...

Aspartyl protease from Trichoderma harzianum CECT 2413: cloning and characterization.

A gene that encodes an extracellular aspartyl protease from Trichoderma harzianum CECT 2413, papA, has been isolated and characterized. Based on several conserved regions of other fungal acid proteases, primers were designed to amplify a probe that was used to isolate the papA gene from a genomic library of T. harzianum. papA was an intronless ORF which encoded a polypeptide of 404 aa, includin...

In vitro Studies on Biodegradation of Chlorpyrifos by Trichoderma viride and T. harzianum

Although a wide application of pesticides and herbicides are an essential part of augmenting crop yields, excessive use of these chemicals leads to the microbial imbalance of soil, environmental pollution and health hazards to human and animals. An ideal pesticide should have the ability to destroy target pests quickly and should get degrade the toxic and nontoxic substances as quickly as possi...

Suppression of Pratylenchus penetrans and Tylenchorhynchus dubius by Trichoderma viride.

Trichoderma spp. are common soil fungi which influence tile development of plant-pathogenic fungi in the soil (1, 8). Yang et al. (9) found that T. harzianum influenced the severity of Fusarium wilt of cotton in the presence of Meloidogyne incognita. We report herein that the survival of two other plant-parasitic nematodes also was influenced by addit ion of cornminuted cultures of Trichoderrna...

Trichoderma harzianum peritonitis in peritoneal dialysis.